Ep, epipodite; En, endopodite; Ex, exopodite; Fc, filter comb.
Similarly 24 are increased at 7 DPP and 6 increased at 21 DPP with a log2 FC filter of >1.5 in CE (Additional file 7: Table S7.2).
This number is reduced to 14 and 3, respectively, when a log2 FC filter of >1.5 is applied to exclude lowly differentially expressed miRNA (Table 2).
Two miRNA are increased in expression at 7 DPP and 6 are increased at 21 DPP with a log2 FC filter of >1.5 in HC (Additional file 7: Table S7.1).
Selection of differentially expressed probesets (DEPS) between the replicates of tumor and normal samples was performed using the following criteria: (1) Fold change (FC) filter: |log2 FC)| > log2(1 + 3* μCV) = log2(1.2), where μCV is the median of the coefficients of variation of expression intensity (CV i ) for all probesets on the array.
Of these, 4,472 probe sets were differentially expressed across the regions after the Fisher protected LSD tests and fold-change (FC) filtering.
Using a low stringency (p < 0.05 and FC > 1.4) and a high stringency (p < 0.01 and FC > 1.5) filter, the resulting gene sets of 185 and 55, respectively, showed clear separation of cases and controls by PCA as well as 100% classification accuracy by SVM, indicating that the gene profiles can separate patients from controls.
Current records were filtered using an eight-pole active Bessel filter (fc, 0.3 kHz) and clamp current and voltage data were digitized at 1 kHz.
Data were reconstructed using filtered back projection and a Butterworth postreconstruction filter (Fc 0.23; order 6), using Segami software (Segami, Columbia, MD).
Differential expression was tested by one-way anova without equal variance assumption (Benjamini Hochberg multiple test corrected P ≤ 0.01), followed by post hoc Tukey HSD (P ≤ 0.01) and fold change filter (FC ≥ 1.5) between conditions.
