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We analyzed rabbit Fc protein (Fc) and Fc-fused HrTCTP by Western blotting using rabbit IgG specific antibody.
The resulting antibody derivative mediates Fc receptor binding by virtue of the Fc protein and selectively targets cancer cells expressing human integrin α4β1.
Cells treated with Fc protein or Fc + G-CSF were used as controls.
Wells coated with an equal concentration of the Fc protein were used as negative controls.
Leptin receptor was used as Fc protein and binding specificity control.
Human IgG1 Fc protein was purchased from Accurate Chemical and Scientific Corporation (Westbury, NY, USA).
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In contrast, among the Fc protein-treated cells the CD11b+, CD14+, F4/80+ populations were declined substantially.
At day 14, granulocytes among the Fc protein-treated cells remained at 9.6%, whereas they were not detectable (0.7%) among the CD137 protein-treated cells (Figure 1A,B).
For all of the above experiments we had used human CD137-Fc protein.
Moreover, we treated both L1−/y neurons and L1+/y neurons with L1-Fc protein.
The endotoxin concentration in the CD137-Fc protein is 55 IU/mg.
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