The fibrillar centre (FC) is enriched in components of the RNA pol I machinery.
A comparison of standard FC and double labeled FC, using samples enriched with cancer cells, showed that many BAC that were diploid by standard FC were found to be aneuploid by the double-labeled FC [ 10].
No clearer example exists for the importance of Asn297 to the function of the Fc than the recent demonstration that sialic acid enriched Fc-fragments are responsible for the beneficial effect of intravenous immunoglobulin (IVIG) therapy in preventing autoimmune disease (Anthony et al, 2011).
The Rosa26BAC CAGGS Fc culture was enriched twice using eYFP FACS sorting.
Certain Fc glycan variants enriched for terminal sialic acid render IgG overtly anti-inflammatory, accounting in part for the action of high-dose intravenous Ig [ 3, 4].
IgG1 Fc glycopeptides were enriched and desalted by reverse phase (RP) solid phase extraction (SPE) using Chromabond C18ec (C-18) beads (Marcherey-Nagel, Düren, Germany).
Finally, the Fc-library was probed for binding to structurally specific ligands, followed by flow sorting and after 4 consecutive sorting rounds enriched Fc variants were sub-cloned and expressed solubly in Pichia pastoris.
Enriched Fc-mutants were sub-cloned from pYD1 into pPICZαA (Invitrogen, Carlsbad, CA, USA), a vector suitable for soluble expression in P. pastoris.
With both stoichiometric and excess DMPC, increasing the level of FC to 15 mol % resulted in larger rHDL, enriched in FC.
This analysis revealed that predicted CRMs associated with Slou-responsive FC genes are equally enriched for Slou-preferred sites, together with Pnt and Twi, as the previously delineated combination of FC regulators, Pnt, Twi and Tin (Fig. 5A; supplementary material Table S5) (Philippakis et al., 2006).
In particular, DEGs most strongly elevated in PP skin (FC > 3) were enriched for genes highly expressed in KCs and epidermis.
