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Considering the much larger increase in polyadenylated transcripts, it is tempting to speculate that heat treatment not only increases transcription but also the level of faulty transcript synthesis that leads to enhanced polyadenylation activity.
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Although NMD mainly acts as quality control to eliminate faulty transcripts in gene expression, it is also involved in physiological and pathological functions [ 68, 69].
NMD is a complex process in mammalian mRNA metabolism, and its function is to eliminate faulty transcripts and control the expression of normal genes.
The faulty records system generated inaccurate transcripts and miscalculated grade-point averages, among other problems.
It is also possible that intron retention is a result of a faulty splicing event, and such a transcript, with a premature termination codon (PTC), is destined to degradation via the nonsense-mediated mRNA decay (NMD) pathway [ 47].
Werner et al. used these faulty arrays to detect the expression of antisense transcripts in mouse brain and kidney [ 14].
These faulty cap structures can be removed by the decapping endonuclease Rai1, resulting in a 5′-monophosphorylated transcript that is susceptible to degradation by Xrn2 [ 56].
It is important that the cell identifies and removes these faulty polypeptides so as to leave the ribosome free to translate other (non-faulty) mRNA transcripts.
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