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At the end of 144th hours, total dry cell weight, total fatty acid amount and DHA amount were calculated as given in Fig. 2.
Figure 2 shows the total dry cell weight, total fatty acid amount and calculated amount of DHA based on fatty acid composition percentages obtained from GC-FID analysis at the end of 144th hours.
Due to variations between clones, among all the 14 yeast strains harboring cocoa genes, only YJ-212 (harboring the combination of TcGPAT2, TcLPAT1 and TcDGAT2) and YJ-221 (harboring the combination of TcGPAT2, TcLPAT2 and TcDGAT1) displayed a significant increase (29.1 and 9.9%, respectively) of the total fatty acid amount compared to YJ0 (Fig. 4).
Individual fatty acid amount was measured by GC-MS and normalized to liver weight (nmol/g).
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In our study, we were also considered that the above-mentioned factors influence the distinctions in fatty acid amounts.
We found that fatty acid amounts of neutral lipids are increased in liver of LPCAT3-deficient mice.
10.7554/eLife.06328.015 Figure 5. Arachidonate levels and metabolism are changed in LPCAT3-deficient mice GC-FID was performed using various tissues to analyze fatty acid amounts and compositions in total lipids, as well as those of lipid subclasses.
A similar experiment was performed using proximal small intestine, and fatty acid amounts of phospholipids are decreased in this tissue of LPCAT3-deficient mice, consistent with the decreased PC levels.
A lower absolute (mg/L plasma) and relative (% of total fatty acids) amount of DHA in maternal plasma phospholipids during pregnancy was seen in multigravids compared to primigravids [ 22], suggesting incomplete recovery of maternal DHA availability after delivery [ 23].
With 240 g l-1 sucrose only 13.5% of the cellular dry weight consisted of fatty acids, whereas in presence of 20 to 40 g l-1 sucrose fatty acid contents amounted up to 45.1%. Figure 2 Growth of R. opacus under different nutrient concentrations and pH values.
As to the contents of the pool of free fatty acids, the amount of unsaturated fatty acids with carbon chain length of C16 and C18 was significantly higher in the slr1609 knockout mutant strain compared to the wild-type strain.
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