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A highly versatile technique has been developed in mouse: MASTR (mosaic mutant analysis with spatial and temporal control of recombination), which utilizes the increasing number of floxed alleles and simultaneously combines conditional gene mutagenesis and cell marking for fate analysis.
Coronal sections (n = 3 per group) were collected at 50 µm for fate analysis.
From the 1 in 12 sampling of sections stained, the ten consecutive sections that contained the most human cells were utilized for fate analysis.
By focusing on the lungs of the conditional mutant embryos and combining fate analysis and global gene expression pattern studies, we show for the first time that mesenchymal ß-catenin signaling has a dual, lineage-dependant function.
Sub-epithelial cells express Ptch and respond to epithelially-derived SHH, while transient fate analysis studies using an Fgf10-LacZ reporter line (here after termed Fgf10LacZ/+), show that the sub-mesothelial cells express high levels of Fgf10 and serve as progenitors to parabronchial smooth muscle cells (PSMCs).
Fate analysis revealed SC activity near regenerating NMJs.
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Elimination baseline Wnt activity, via application of an extracellular Wnt antagonist promoted neurogenesis, based on a combination of unbiased gene expression analysis and cell-fate analysis.
The study hereby described follows on from the earlier work of the authors and aims at performing a source-pathway-fate analysis of lead (Pb) and polycyclic aromatic hydrocarbons (PAHs) in the urban soils in order to increase current knowledge on factors influencing exposure of the population.
Ginhoux, F. et al. Fate mapping analysis reveals that adult microglia derive from primitive macrophages.
Narni-Mancinelli, E. et al. Fate mapping analysis of lymphoid cells expressing the NKp46 cell surface receptor.
We next asked whether these three populations derived from one another and used fate mapping analysis to establish their lineage relationship over time.
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