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The XPS spectra were recorded using a PHI 5000 Versa Probe (Chanhassen, MN, USA), with a scanning ESCA microscope (Trieste, Italy) fitted with an Al monochromatic X-ray source (1486.6 eV, 25.6 W), a beam diameter of 100 μm, a neutralizer at 1.4 eV 20 mA, and in FAT analyzer mode.

Percentage of body fat was estimated by bioelectrical impedance method (Omron Body Fat Analyzer HBF-306).

Body fat percentage is measured with a body fat analyzer device (TANITA) [ 32].

Body fat was measured using an impedance method (TANITA body fat analyzer; Tanita Corp., Tokyo, Japan).

Body composition was assessed using bioelectrical impedance analysis (Tanita 305 body fat analyzer; Tanita, Tokyo, Japan).

Weight was measured using Tanita scales Body Fat Analyzer model TBF 305 (Arlington Heights, IL).

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Body mass and composition were estimated using bioelectrical impedance (TBF-300 Body Fat Monitor/Scale Analyzer, Tanita, Tokyo, Japan) floor scale with an accuracy of 0.1 kg.

As regards bioanthropometry, patients were measured for body weight, height, body mass index (BMI), relative and total body fat (BodyFat Analyzer, Omron BF 500), waist circumference and conicity index (waist circumference (m)/0.109 √[weight (kg)/height (m)]) [ 27- 29].

Adiposity is often estimated through body mass index (BMI), but it can also be appreciated through determination of other fat-mass indicators, such as waist circumference, skinfold thickness and percent body fat, estimated with a bioimpedance analyzer.

Crude fiber was analyzed by FOSS analyzer (Fibertec™ 2010, FOSS GmbH, Rellingen, Germany) and crude fat according to the Soxhlet procedure (method no. 5.1.1).

Bio-electrical impedance analysis (BIA) was completed by employing a Maltron Bioscan 9200 standard tetra-polar multi-frequency analyzer (Maltron International, Rayleigh, Essex, UK) that analyzes the percentage fat, fat mass, body cell and muscle mass.

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