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S. cerevisiae strain D452-2 (MaT a leu2 his3 ura3 can1) (Kurtzman, 1994) and SR8U (the uracil autotrophic version of the evolved xylose fast utilization strain SR8) (Kim et al., 2013) were used as recipient strains for the yeast experiments.
Hence, aside from small positive contributions made by XDH to qxylose, high activity levels of XR paired with high flux capacities of UG are essential for CBS4435 to keep Yxylitol at low levels while permitting fast utilization of xylose relative to that of BP000.
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These variables may be explained by the higher bioavailability of OTM and faster utilization and fermentation by rumen microorganisms.
The faster utilization of sugar and removal of furaldehydes from the liquid phase by adapted cultures (Fig. 8) demonstrates that the ability of the yeast strain to tolerate and transform inhibitors improved with short-term adaptation.
Furthermore, there have been changes in metabolism as well, such as reduced expenditure of metabolized protein and faster utilization of ingested lipids (Krasnov et al., 1999), along with improved food conversion ratios (Rahman et al., 1998).
Genomic sequencing of the evolved strain with fast xylose utilization capability was performed, and mutations possibly affecting xylose utilization were screened.
Highest productivities were obtained by using the AOX promoter with a fast methanol utilization strain (Mut+) of P. pastoris.
S. cerevisiae is adapted to nutrient rich environments in which it has competitive advantage from fast nutrient utilization and a high rate of ATP production through the fermentative pathway, whereas T. reesei is adapted to nutrient poor environments where it benefits from high energy yield [ 17, 16].
After three rounds of ALE, two selected mutants of ZMF3-3 and ZMA7-2 demonstrated higher growth capacity and faster glucose utilization than wild-type ZM4 under 3 g/L furfural or 7 g/L acetic acid stress conditions.
These results further affirm the role of unknown cell wall component(s) in the sensing of competition by PP9 thereby, leading to higher TOL expression and faster BA utilization.
Strain IBB10B05 showed the highest μGlucose and the fastest glucose utilization.
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