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A fast SHAKE algorithm to solve distance constraint equations for small molecules in molecular dynamics simulations.
Kräutler, W.F. van Gunsteren, P.H. Hünenberger, A fast SHAKE algorithm to solve distance constraint equations for small molecules in molecular dynamics simulations.
Kräutler, V., van Gunsteren, W. & Hünenberger, P. A fast SHAKE algorithm to solve distance constraint equations for small molecules in molecular dynamics simulations.
In this study, a fast shake mixing control system that allows control of the quantity of air entrainment, involving low mechanical stress and fluid bubbling by means of the suppression the sloshing of a fluid's surface, is developed.
Thermal motion of water molecules also contributed to fast "shake off" of the ligand copies especially in the cases of Systems with small ligands with the application of the simulated annealing protocol (MDBSA, see an SR of 23 in case of System 2 in Table 2).
The plates were then placed in a Micro plate Reader (Synergy H1Multi-Mode, Biotech, Winooski, VT) for absorbance (OD600 for cell growth) and GFP signal (filters with 485 nm excitation and 535 nm emission for protein expression) measurements every 5 min for 2 h after fast shake for 1 min. All tests were performed in the dark in triplicate.
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The artists – Uhuru B, Swift, Smokillah, Bankslave and another man who prefers not to be named – work fast, shaking, spraying, surveying.
As a young airline that was scrutinized for growing too fast, shaking the crash's stigma was more difficult than for other carriers.
She continued: "Dream as if you will live forever, and live as if you will die today". Andy blinked fast, shook his head and said: "I like that quote". James got chills.
OD600 was measured at 15 min intervals for 15 h at 34 °C with fast shaking mode on a Synergy2 plate reader (Biotek, Winooski, VT).
Following 10 min incubation at 37°C, the infected culture was diluted into 400 ml LB (supplemented with 0.1% Glucose, 1.6 mM MgCl2, 0.5 mM CaCl2) and incubated at 37°C with fast shaking and good aeration.
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