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Step-One real-time PCR system was used for quantitative RT-PCR and the reaction was performed with the PerfectaSYBR green Fast mix ROX (Quanta).
qPCR was performed with Syber Green fast mix (Quanta Biosciences, Gaithersburg, MD, USA) using the Biorad CFX96 Detection System instrument and software (Bio-Rad Laboratories Ltd, Hempsteadpstead, UK).
Repeat the steps backwards, and get the hang of mixing it up and fixing it, once you can do it really fast, mix it up, invite your friends over, and solve it.
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Previous studies on the mixing time of the hard-core model investigate the conditions of fast mixing.
The fast mixing process is the key advantage of micromixers.
Robotic component of Tracer-QC system allows for precise and fast mixing of the liquids.
To design a fast mixing control system, the hybrid shape approach was applied.
The fast mixing within micromixers leads to high supersaturation of the vapor and to homogeneous nucleation.
In fact, since precipitation processes are generally very fast, mixing plays a crucial role and it is of great importance to operate under very fast mixing conditions.
A fast mixing nozzle has been designed to generate the complex preventing the reaction between the components.
The mixing time of 160 ns is at least 50 times shorter than estimated for other fast mixing devices.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com