Similarly to other sequential cardiac imaging methods, multiple fast image series are consecutively acquired for different sections but in between acquisitions, the imaging plane is rotated around the center line instead of shifted along the direction perpendicular to the slices.
In contrast, our method uses spinning disk confocal microscopy for optimal fluorescent imaging resolution and to provide fast image acquisition rates required for live cell imaging, able to capture 15 20 full frame images per second.
The data were collected using OPUS 5.5 (Bruker Optics, Germany), which has a kinetic imaging mode for fast image capture of the segmented flow system.
This modality promises fast image acquisition, good resolution and contrast, and inexpensive instrumentation for imaging of weakly scattering objects, such as for example tissue-engineered constructs.
Previously, we reported applying SRS to the imaging of lignin in plant cell walls, with spatial resolution and fast image acquisition based on lignin's unique strong aromatic Raman vibration band at 1600 cm−1 [ 15– 15].
Parallel spectral imaging, on the other hand, samples fluorescence simultaneously onto several detectors allowing fast image acquisitions, a prerequisite to follow fluorescence transients.
The ease of access, fast image acquisition time, thin section scanning and multiplanar reformations make MDCT the ideal imaging modality.
Fast image reconstruction is provided by GPU processing.
They also provide high counting rates with fast image readout.
In this paper, a fast image encryption algorithm with combined permutation and diffusion is proposed.
