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In this study, P. chrysogenum intracellular metabolites were quantified directly from biomass extracts using automated sampling and fast filtration.
And when a movie connects with a crucial niche audience, the fast filtration (thanks to the world of the Internet) from the margin to the mainstream may well effect changes in popular attitudes not because of any political persuasion or underlying social change, but purely because of the artistic equivalent of fashion and style that would otherwise have arrived only very slowly and very gradually.
The crystals formed (UCF) were separated from the liquid (NUCF) by fast filtration.
In case of small polar compounds (amino acids, organic acids, and small phosphorylated metabolites), a cell amount corresponding to 0.6 mg cell dry weight was sampled by fast filtration [51].
Culture samples of 2 7 mL, equivalent to about 109 cells mL−1, were harvested and separated from the media by fast filtration in the light using a glass vacuum filtration device with controlled temperature and illumination.
The first step is a fast filtration of the unreliable regions for all possible oligos using a seeding algorithm.
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The procedure features a good and adjustable level of grafting, a high reproducibility, fast filtrations, and a scalability on a gram-scale.
The proposed system was further simulated for minimum and maximum values of the collimator ratio for different sapphire filter thicknesses which is commonly used for fast neutron filtration.
The radioactivity concentration of the low molecular weight fraction of venous plasma samples separated by fast gel filtration was used as input function.
Coarse particles and adhering mycelium were removed with a sieve (mesh size 2 mm), residual solids were then separated by fast vacuum filtration using a Whatman disc filter with a pore size of 8 12 μm.
For preparation of metabolite extracts, the cultivations were sampled two (wild-type experimental design cultivation samples for LC-MS/MS analysis) or three (for all other conditions) times for each of the two MS-methods (biological replicates) to be employed, using fast vacuum filtration and quenching of metabolism in 37.5% cold methanol (-20°C).
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