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Fermentas Fast end repair (15 min RT), Klenow exo− (3 μl, 30 min at 37°C) and fast ligase (1 μl, 10 min RT) were used for library construction in 50 μl volumes, with a single PBS wash between each reaction.
Other stories live on the fast end of the scale.
At the fast end, that is more than enough to transmit two high-definition movies a second, from each of 96 ports, or data outlets, on a single switch.
An outlier on the fast end can improve his stock and an outlier on the slow end can hurt his, but in the vast middle, it's not all that important.
This transcendental folk, deconstructed by the volatile rhythms of post-rock, demands to be danced to, and when the music hit the loud and fast end of the spectrum – an all-out head-banging mosh.
It was inaugurated on the premise of flawed idées fixes: that it would have "a clean, fast end" and would lead to a democratic regime that would transform the Middle East in a positive fashion.
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Some manufacturers and retailers should be willing to pay a premium for a shipping service that guarantees fast, end-to-end delivery, says George Stalk of BCG.
Other players find it impossible to imagine, said Abdullah, whose Ramadan fast ended yesterday.
Companies that sell bandwidth are now hatching plans to sell really fast end-to-end connections to the people most likely to buy them.
Nielsen knows that the diary era is fast ending.
That fast ended when Chavez shared bread with Sen. Robert F. Kennedy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com