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Furthermore, due to the very fast detachment from pretension AM.ADP.Pi states, suggested by single molecule studies [ 182] and the associated weak actin affinity of these states, it seems unlikely that prepower stroke AM.ADP.Pi states can account for the high resistance to slow stretch.
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Our model supports the notion that faster motility on very stiff ECMs (Fig. 5 A) arises from higher activation levels of Rho and Rac signals (Fig. 4 A,E), and larger adhesions and contractile forces in the front (Fig. 4 C,G) that induce faster detachment in the rear.
High ATP concentrations therefore lead to a faster detachment of myofilaments from actin filaments than at low ATP concentrations (Movie 5).
Interaction with kinetic site 1 is characterized by relatively fast attachment and slow detachment, whereas attachment to and detachment from kinetic site 2 is fast.
These highly economical nurseries (US$0.11/fragmentt) revealed high survivorship low detachment and fast growth rates compared to previous coral-nursery types.
Additionally, we separated drop-size-velocity relationship into lower left and upper right quarter to investigate the effect of small and slow just as big and fast raindrops on splash detachment amounts.
The rate of cortical detachment is fast for Bni1p and very slow for Bnr1p [24].
These results are therefore consistent with the idea that inactivation of the MAGUKs is required to ensure a complete, fast and efficient cell detachment during apoptosis.
The inital slow phase of myofibrillar force relaxation occurs when all sarcomeres remain isometric, whereas during the subsequent fast phase, cross-bridge detachment is accelerated by rapid lengthening of single sarcomeres (21, 26).
Collectively, these results suggest that for a fast and efficient cell detachment during apoptosis the components from the both sides of the membrane have to be cleaved, including the MAGUKs, which appear to be cleaved before the exposure of PS on the outer leaflet of plasma membrane, and before detachment of the cells from the substrate.
Therefore, it can be suggested that, besides ensuring fast and efficient cell detachment, the cleavage of MAGUKs is probably important also for shutting off or disrupting the communication between the dying cell and its neighbors, as the MAGUKs are important scaffolding proteins for the different signaling pathways.
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Justyna Jupowicz-Kozak
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