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Second, to avoid a shift in expression within a sampling time-point due to the delay between the first and last collected samples, we ensured fast sampling by marking beforehand each plant and leaf to be collected.
We divided subjects into child (6 10 years of age), teen (11 18 years), and adult (≥ 19 years) age groups and restricted our analyses to subjects in the morning sampling session who fasted for ≥ 8 hr beforehand.
The subjects were given information on the study protocol 3 days beforehand and were forbidden to exercise, drink alcohol, overeat, or fast during the 48 hours preceding the trial.
That is changing fast.
How did you feel beforehand?
The interventions were standardised beforehand.
They were not informed beforehand.
The method was validated beforehand.
A stopping boundary was not determined beforehand.
All had completed the EDAQ beforehand.
But we plan for this beforehand.
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