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The small thermal mass of the chip, in combination with the low thermal diffusivity of the polymeric substrate on which the heating elements reside, yields a low power consumption PCR chip with fast amplification rates.
After applying a fast amplification protocol to the specific nucleic acid sequences (30 min), a small amount of amplicon was added to the CNP neutravidin suspension, and it was then run on the prepared NALFIA.
If these sequences are integrated into an actively transcribed region, subsequent events such as recombination and gene conversion can spread this repeated sequence, resulting in fast amplification of the original ORF [ 3].
Fast amplification parameters were as follows: one cycle at 95 °C for 10 min, followed by 40 cycles at 95 °C for 15 s, and 60 °C for 1 min.
The key features of our model which we shall explain in more detail below are: 1) slow inhibition due to negative feedback from inhibitors (R), 2) fast amplification due to positive feedback from cytokines (T) and 3) spatial coupling of the NF- κB-like circuits due to diffusion of extracellular cytokines.
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The sensor includes a novel highly sensitive and fast analog amplification circuit in combination with gold electrodes fabricated in PCB technology.
Highly localized and very fast electron amplification of microchannel plates (MCPs) enables a large number of high-resolution and high-sensitivity detection technologies, which provide spatial and/or temporal information for each detected photon/electron/ion/neutron.
Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP).
Region IIIa occurs only when the virus and the silencing response are fast, with amplification these conditions are more likely to occur.
Recently, insulated isothermal PCR (iiPCR), based on Rayleigh-Bénard convection PCR [ 43], has been shown to achieve relatively consistent and fast target amplification by convective PCR.
Here, we assess the usefulness of a recently described in our group LM-PCR method, termed fast ligation amplification polymorphism (FLAP), for differentiation of M. tuberculosis strains [ 15].
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