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A focused laser beam is employed as a reference for automatic and fast alignment of the AFM cantilever tip with the micro-tool cutting edge.
The use of the Pyrex-SU-8 clamping substrate allows an easy replacement of the cantilever chips and a fast alignment process to the DVD-ROM laser beam.
The created vacuum enables fast alignment of high-AR AuNRs on a large scale.
Thus, the size of the ODN is a critical parameter to guarantee a fast alignment between the two molecules.
The estimated scale of the problem aligning a billion reads per day on a moderate computer cluster obviously demands extremely fast alignment algorithms, but more importantly demands high accuracy of the alignments (i.e. obtaining the true alignment of a read, among the many various candidates).
Our fast alignment method relies on fast rotational matching.
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Briefly, Sailfish is an extremely fast alignment-free method for quantifying transcripts (11), with similar accuracy as for example the Cufflinks pipeline (10).
This may be explained by a larger percentage of the k-mers mapping to fewer candidate taxonomic labels, as this is the primary run time cost associated with LMAT classification and could provide a speedup advantage over even fast alignment-based searches using Bowtie2.
The exhibition labels avoid hard-and-fast alignment of peoples, places and styles.
Hence, larger responses lead to a faster alignment between the clock phase and the LD cycle.
At a rotational sampling of 45°, the speedup leads to an 84 fold faster alignment.
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