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The myosin motor domain not only strongly enhances the production of fused proteins but is also used for a fast affinity purification step through its ATP-dependent binding to actin.
The fast affinity screening results included the discovery of a lipid phosphatase inhibitor involved in type 2 diabetes [ 64].
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The products secreted by infective stages are especially relevant in this context: parasite establishment relies, to a great extent, on the capacity of these molecules to give rise to fast, high affinity interactions with their host counterparts.
At crayfish neuromuscular junctions, basal transmission and facilitation were significantly reduced after presynaptic introduction of "fast" high-affinity calcium buffers, and the decay of facilitation was accelerated.
Since the calcium binding sites in C2A may interfere with copper binding we sought a high affinity, fast labelling chelator.
Trypsin was purified from the digestive system of carp Catla catla (Hamilton) by ammonium sulfate fractionation, diethylaminoethyl-cellulose column chromatography, and Benzamidine Sepharose 4 fast flow column affinity chromatography.
CNBD-A provides a high-affinity (slow disassociation) site for cGMP whereas CNBD-B has a lower-affinity (fast disassociation) site.
A structural and functional relationship between the bacterial T3SS and eukaryotic apoptosis was identified using our FAST-NMR ligand affinity screen in combination with a bioinformatic analysis based on our CPASS program.
A structural and functional similarity between PrgI, a type three secretion system protein, and Bcl-xL, a member of the Bcl-2 family of proteins involved in eukaryotic apoptosis, was identified from a FAST-NMR ligand affinity screen in combination with a bioinformatic analysis.
The nascent adhesions that reside near the leading edge have short lifetimes, and bind relatively low-brightness particles, presumably monomers with relatively low affinity (faster fluctuations).
Clarified cell lysates were incubated with 0.3% (v/v) polyethylenimine while being gently stirred at 4 °C followed by centrifugation at 25000 g for 60 min. The resulting clarified supernatant was diluted 2-fold in lysis buffer and then incubated with Glutathione Sepharose 4 Fast Flow GST-affinity resin (GE Healthcare Bio Sciences, Piscataway, NJ) for overnight binding at 4 °C with gentle stirring.
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