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"There's been vague evidence for out-flows, but this is far more quantitative and prescriptive".
We have updated the manuscript to include far more quantitative analysis, see replies to Reviewer 2 comments 1-4 above.
This method of fragmentation provides more reproducible coverage because all the ions are fragmented each run, and the identified peptides are not limited by which precursor ions are select for tandem MS. Furthermore, since only one fragmented ion scan is performed for each survey scan, there is far more quantitative data for the parent ions.
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We chose to follow up the Northern analysis above with the far more sensitive quantitative RT-PCR analysis and to combine this with an Actinomycin D assay in order to evaluate stability of the messages over time.
We will focus on categorical outcomes because they are far more common than quantitative outcomes in the criminal justice applications emphasized here.
While some of the resulting (literature or experimentally derived) concentration values for many of these compounds might not be appropriate for routine clinical studies, they do provide a far more complete and quantitative picture of the plasma/serum metabolome than has previously been achieved.
With the release of the Human Metabolome Database (HMDB) [4], [5] and other related compound or spectral resources such as KEGG [6], LipidMaps [7], PubChem [8], ChEBI [9], MMCD [10], Metlin [11] and MassBank [12], we believe the field has taken an important step towards making metabolomics studies much more quantitative and far more expansive in terms of metabolite coverage.
The Fed can get rates down by applying the policy of quantitative easing far more aggressively.
It is generally accepted that underreporting of this form of violence in quantitative research is far more likely than overreporting [ 20].
The effects of single pollutants are generally inferred from such variations, but characterization of quantitative relationships is far more imprecise for the effects of combinations of pollutants than for the effects of single pollutants.
Unfortunately, many things in life are qualitative, not quantitative, and are far more complex than this, so trust your heart and don't fall prey to oversimplification.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com