In this proof-of-principle methodology, as low as 50 nM of a target DNA was detected, although we envisage far-lower detection limits.
This variation detected by HRM was far lower than the detection from amplicon sequencing of sugar cane though the sugarcane work focused on genes of interest whereas we used a de novo approach [ 15].
This is far lower than the detection limit required by the European Union directives for drinkable water and food samples (0.1 μg L−1).
However, we have found when analysing crude extracts that the selectivity of the negative ion mode gave a far better sensitivity and therefore lower detection limits.
But this detection rate was far lower than that in electron microscopy or immunofluorescence using genus-specific antibodies, which showed Chlamydia spp. in over 80% [ 17].
It has been shown that biopsies that are not guided by a 3-D imaging approach of a suspect lesion have odds of detection that are far lower than a clinician might expect [ 10].
The detection limit of BMQ for Cu2+ was far lower than the maximum allowable level of the U.S. Environmental Protection Agency limit (20 μM) for drinking water.
Using the fluorescence spectra changes, we found a detection limit for CN− of 0.15 μmol L−1, far lower than the maximum permissive level of CN− (1.9 μmol L−1) in drinking water recommended by World Health Organization (WHO).
The detection limit for CN− was 4.0×10−7 M, which is far lower than the WHO guideline of 1.9×10−6 M.
The detection limit (0.32 μM) of 1 for Zn2+ was far lower than the guideline of World Health Organization in drinking water. 1 was used to determine Zn2+ concentration in various water samples.
The excitation and emission optima require appropriate filter sets, although signal detection with commonly available filter sets is possible, though with far lower sensitivity (see Fig. S2).
