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So far, binding to a Rho-type GTPase like Rac has not been reported for Als2cL.
During visual inspection of all potential domain-peptide interactions we identified 7 distinct motifs in interactions of known structure that did not match any of the patterns collected in ELM so far, binding to the domains 14-3-3 14-3-3 14-3-3 new MATHfs) and SH2 (2 new motifs).
A total of approximately 80 compounds has been studied thus far Binding to DNA of rodent tissues was readily detected by the 32P-postlabeling assay for all known carcinogens among these compounds, and adducts were detected in DNA from human placenta of smokers.
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However, so far, no binding to fungal or bacterial cell walls was detected (Frischmann et al. 2013).
Of all the compounds tested, DAS534 has the largest van der Waals surface area and was also the strongest by SPR assay with far stronger binding to AFB5 than IAA.
In the metazoans studied thus far, binding of HSF or HSF1 to HSEs is low to virtually nonexistent in unshocked cells and upon HS or other stresses, HSF converts from a monomer to a trimeric form that binds to the HSEs with high affinity.
The majority of SH3 domains studied thus far act by binding to proline-rich sequences in partner proteins, but a growing number of studies have revealed alternative recognition mechanisms.
Allosteric modulators have been considered so far as molecules binding to an allosteric site, distinct from that of the reference ligand (orthosteric site), and able to modulate the binding affinity at the orthosteric site and/or the signaling properties resulting from orthosteric site occupancy.
Non-specific binding of TRAIL to RGDPEG-avidin was however far lower than binding to unmodified avidin, most likely due to the PEGylation (data not shown).
Cell death and apoptosis were tracked via the nuclear incorporation of propidium iodide (PI; near red; all forms of cell death) and AlexaFluor647-conjugated AnnexinV (far red; apoptosis) binding to the outer membrane (and eventually entering the cell).
High level of SPIONs-CA-FA binding to FAR + cell lines was assured via qualitative and quantitative in vitro binding studies.
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