Exact(50)
Now open your igloo and go to the pet shop and say FAM FAM AT (penguins name here) ON THE MAP! Do this for three to five minutes in the pet shop.
GLASTONBURY GONNA GET BURIED AFTER WE SHOW THE FAM AT THE FARM HOW HARD LAZER FANS GO. — MAJOR LAZER (@MAJORLAZER) June 28 , 2013Other stage, 10.30pm They've cleared an extra camping field to accommodate the expected crowd for the Rolling Stones, but for anyone on site wanting respite, the high energy of Chase & Status on the Other stage at the same time won't necessarily offer it.
Individual assays (Table 2) were designed with the aid of Genscript real time PCR design software (VWR, West Chester, PA) and synthesized by Operon Biotech, Inc. (Huntsville, AL) with 6-carboxyfluorescein (FAM) at the 5' end and FAM-BHQ1 quencher at the 3' end.
FAM-apt is characterized by absorption bands of the DNA sequence (260 nm) and FAM (503 nm), whereas the addition of GO into the solution of FAM-apt causes a red shift and the absorbance of FAM at 503 nm is increased.
As substrate for ssDNA a 15 nt dual labelled oligonucleotide was used (5'-FAM - CGC CAT CGG AGG TTC- BHQ1-3' ), whereas a dsDNA substrate was prepared by mixing equimolar amounts of two complementary oligonucleotides labelled with FAM at the 5'-end or with a BHQ1-quencher in the 3'-end, respectively (5'-FAM - CGC CAT CGG AGG TTC -3'/5'- GAA CCT CCG ATG GCG BHQ1 -3' -).
In this paper, the mechanical responses of FAM at different volumetric compositions are systematically studied.
Similar(10)
The FFAM-apt with a sequence of 5′-FAM-AT CTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGA-3′ was synthesized by the Sangon Biotech Co., Ltd.
However, ZIF-8/5-FU@FA-CHI-5-FAM exhibits a stronger cell growth inhibition effect on MGC-803 cells compared to ZIF-8@FA-CHI-5-FAM at the same concentration.
The substrate strand was labeled with a 6-carboxyfluorescein fluorophore (6-FAM) at the 5′-end and a Cy5 at the 3′-end (6FAM/Cy5-17DS 6FAM/Cy5-17DS
The forward primer was fluorescently labeled with the 6-FAM at its 5′ extremity.
All oligonucleotides are labelled with 6-FAM at 3' and 5' ends.
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