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Closer analysis of the positions also ruled out false variations caused by too high coverage.
False variations may arise, in part, from the incorrect mapping of reads.
Too high sequencing depth increases noise and could have introduced, without very strict quality control, false variations [ 38].
To follow patients longitudinally, it was essential to prevent minor wrist or hand rotation from session to session which might cause false variations in volume measurements.
This process automatically compensates for missing data in some monitors on individual days by preventing that missingness from contributing to false variations in the daily value.
However, in metagenome sequencing, one cannot simply map all metagenomic reads to a reference genome, because some of the aligned reads may originate from different microorganisms, which could introduce false variations.
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This result was expected, since SNPs&GO was tuned to have a very low rate of false positives (variations predicted as disease but observed as neutral) at expenses of the coverage (some disease related variations are discharged as neutral).
Another kind of small false signal variations can be noise caused by the imaging chip which can be reduced by filtering (as explained in Section 3).
Such an overestimated quality scale prevented the filtration of false-positive variations while GATK runs genotyping.
They also address some of the challenges of GWAS, such as the high rate of false discoveries, variation between patient cohorts, and accessibility of validation cohorts.
This metric advanced the state-of-the-art for resequencing studies because then the variation discovery algorithms and other post-mapping algorithms could use this score to rule out false-positive variations caused by incorrectly mapped reads.
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