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Quantitative assay results falling within the linear range of the assays were reported in copies/mL.
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Most clinical specimens tested (40/41) fell within the linear range of the assay.
Specimens were chosen on the basis of having fallen within the linear range of the first assay.
The number of cycles used was optimized for each gene to fall within the linear range of PCR amplification.
The amount of protein loaded was adjusted for each antibody so that signal intensity fell within the linear range of the assay (Supplemental Figure S2).
Multiple serial dilutions of a reaction were fractionated alongside the undiluted reaction, and the substrate band was quantified from those that fell within the linear range of estimation.
The band intensity in the input lanes and biotin lanes, which fell within the linear range, was quantified to calculate the % of total GluR1 or GluR2 on the surface for each sample.
Samples were diluted appropriately to fall within the linear range of the curve.
The cell extracts were diluted 1 : 5, so that the measured concentrations fell within the linear range of quantification.
48) If a standard curve was prepared, ensure that all samples fall within the linear range of the assay.
When necessary, samples were diluted so that values fell within the linear range of the assay (0 1 µM).
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