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The number of cycles used was optimized for each gene to fall within the linear range of PCR amplification.
Samples were diluted appropriately to fall within the linear range of the curve.
48) If a standard curve was prepared, ensure that all samples fall within the linear range of the assay.
The number of cycles used was optimized for each gene to fall within the linear range of PCR amplification: GAPDH = 18 cycles; CTNNB1 = 23 cycles; PECAM1 = 22 cycles.
The samples were diluted 16-fold before measurement after confirming that the SEAP activities fall within the linear range of the assay.
Ensure all target samples fall within the linear range - any samples that amplify outside this linear range should be removed from further analysis.
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Most clinical specimens tested (40/41) fell within the linear range of the assay.
Specimens were chosen on the basis of having fallen within the linear range of the first assay.
The amount of protein loaded was adjusted for each antibody so that signal intensity fell within the linear range of the assay (Supplemental Figure S2).
Multiple serial dilutions of a reaction were fractionated alongside the undiluted reaction, and the substrate band was quantified from those that fell within the linear range of estimation.
The band intensity in the input lanes and biotin lanes, which fell within the linear range, was quantified to calculate the % of total GluR1 or GluR2 on the surface for each sample.
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