Your English writing platform
Discover LudwigExact(1)
If necessary, faint products or products with multiple bands were cloned with the TOPO TA cloning kit (Invitrogen, Carlsbad, CA) following the manufacturer's instructions.
Similar(59)
PCR products were obtained from a third of the surface bark samples, but only one faint product was obtained from 70 samples taken from beneath the outer bark.
A faint product from this region was also detected in hma2 hmandand hma2 hma4 transformed with the empty vector, indicating low expression of a partial AtHMA4 transcript from this mutant (Figure 2B).
Clone 2A03 was almost exclusively expressed in the prostate, with only a faint product observed in the testes.
Use of the primers with heterozygous mutant Nnt C57BL/6J template produces an additional faint product of approximately 900 bp that assists assignment of genotype.
There was clearly a product for Kir2.1 (lane 5) and a very faint product for Kir2.2 (lane 6) despite a strong signal with the Kir2.2 primers from the genomic DNA template (lane 3).
When the S. typhimurium WaaI was reacted with Glc-Hep2-1-deP-KLA through coupling only to the WaaG reaction, a faint product consistent with Gal*-Glc-Hep2-1-deP-KLA (the asterisk indicates this Gal is added in a linkage different from that of the Gal added by WaaB) is observed, suggesting that WaaI does not require the branched α1→6 galactose added by WaaB.
A small percentage of the samples (10%) had faint secondary products that could not be resolved.
In lane 2, analysis of the nucleoplasm fraction detected only faint PCR products representing unprocessed nucleoplasmic pre-mRNAs from these gene loci.
It can also add a second galactose residue to its first product, though the activity is low; after reaction for 30 min, a faint second product band can be observed.
All the His+ clones resistant to G418, when subjected to PCR analysis using AOX1 primers, gave a faint amplification product (~ 2.2 Kb) corresponding to the native AOX1 gene and a bright amplification product (~1.4 Kb) comprising of SAM2 gene, implying stable integration of recombinant plasmid, containing SAM2, gene by homologous recombination at AOX1 in the genome of P. pastoris.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com