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Only tumour cells staining well above the background level (faint labelling was occasionally observed around strongly positive cells, most likely due to antigen diffusion) were considered to be positive.
Switching the Pex11 GFP-labelled cells from oleate to SD-N medium restores comPex11 GFP-labelledhe two peroxisomal markers: 3 h after switcells most ofrome clustered structures have disappeared, punctate structures are present, and soleateintolabelling of the vacuole became apparent (Supplementary Figure S1E).
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Faint labeling by either antibody that was unmatched by strong labeling of the other antibody was never observed.
At a concentration of 10 µg/ml of Con A there was only a faint labeling of the extracellular matrix and none of the mast cells were labeled (Fig. 4A and B).
In these spermatocytes, CAP-H, as CAP-D2 (Supplementary Fig. S5) and CAP-G, were detected as a faint labeling on bivalents (Fig. 2G and H), and as pairs of bright dots (double arrowheads in Fig. 2G).
In contrast to the findings of Wang et al. (2003), however, we observed faint labeling of PNA-negative synapses with anti-SV2A, indicating that SV2A is expressed in rod synapses.
While it is possible no functional Cav1.4 protein is present in the Cacna1fnob2 mouse retina, the reduced protein levels in the retina may have accounted for the apparent background labeling in previous reports (for example, the faint labeling in Figure 1b from Chang et al., 2006).
Furthermore, a faint labeling of 5HT-ir neurites can be observed in the HEs.
Moreover, numerous EPs exhibited lengthened shapes and faint labeling compared with controls (Fig. 4).
Only faint labeling of GFAP+ radial fibers is observed in the OT of young N. furzeri (Fig. 5A).
All ITCs were strongly immunoreactive, and the prominent labeling of these clusters stood out against the faint labeling of other amygdaloid areas (Fig. 2a f).
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