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Although the adipocyte- and muscle-expressed transgenes showed faint expression in muscle and adipose tissue, respectively, the phenotypes of the mice were very different.
Only four genes were expressed in the larval fat body and just one, Mur24F/Dp, showed very faint expression in hemocytes.
The results showed that PtAP1 was detected in almost all reproductive tissues, but only faint expression in vegetative tissues.
The sprouting vessels in the myocardial infarct showed upregulation and expression of β3 integrin, whereas only a faint expression in the muscle cells in the remote myocardium was observed (Figure 2).
CD59 positive signal was distributed in the gray matter with faint expression in the ependymal cells (Figure 3C).
Consistent with previous studies [21], we observed only faint expression in the trunk and tail of wild-types at 28 hours post-fertilization (hpf) (Fig. 1A).
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In contrast to the faint expression observed in most of the adult tissues, Neat1 was highly expressed in the corpus luteum, and the formation of luteal tissue was severely impaired in nearly half of the Neat1 KO mice.
By 11.5 dpc, strong staining is also apparent in the urogenital sinus and the duodenum with faint expression detectable in the ducts within the liver.
In the sham group, there was only a faint expression of COX2 in CA1, but COX2 expression increased rapidly in hippocampus CA1 following global ischemia.
Furthermore, immunohistological analysis revealed strong overlap of Dsp and Bcl11b expression in the dentate gyrus of wild-type animals at P14 but only faint expression of Dsp in part of Sox2-positive cells.
Analysis on a single-cell level revealed faint expression of 46F-Gal4 in stage 17 embryos (suggesting a late onset of Gal4 expression), which was restricted to ePG2 in the NER and to ePG4 and ePG10 in the MFA (Fig. 2D, n=115 hs/nine embryos).
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