There were not so many cases with intermediate staining intensity, faint staining (n=8) and moderate staining (n=3).
0: negative, 1: faint, 2: moderate and 3: intense.
For each tissue sample, the intensity of the staining of CIS cells, was evaluated and scored as negative (neg), faint staining, moderate staining and strongly positive staining.
For each sample, staining intensity (0, faint to moderate, intense) and percentage of positive tumour cells was estimated.
FOXG1 protein expression was evaluated as 0 (negative), 1+ (faint), 2+ (moderate), 3+ (strong) and 4+ (marked).
The intensity of staining was scored as 0 (negative), 1+ (faint), 2+ (moderate), 3+ (strong) and 4+ (marked).
The staining intensity was estimated semi-quantitatively on a scale of 0 3+ as the following: −, no detectable stain; −/+, faint; +, moderate; ++, strong; and +++, very intense staining, as previously described [ 12].
Among some of the features our cases had in common with M45, the following should be emphasised: i) the molecular protein profile showed a characteristic low molecular weight band under 14 kDa, ii) the cerebellum was the most affected region, iii) PrPSc was mainly detected in the neuropil predominantly as fine granular deposits, and iv) a faint to moderate PrPSc signal intensity was seen.
XIAP was assessed for intensity as 1 (negative or faint), 2 moderate, 3 (strong), with 2 and 3 being positive.
Eight of 29 (27.5%) were faint and 5/29 (17%) were of moderate density.
