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Some agency officials cite an incident in the 1950's when a slight change in the production process led to a failure to inactivate completely the virus used in the Salk polio vaccine.
The aim was to examine the impact of naturally occurring fluctuations in suspended solids concentration on failure to inactivate viable E. coli.
Notably, this failure to inactivate the critical cellular targets and interrupt phagocytosis occurred despite efficient antiphagocytic Yop effector translocation into the target cells.
The delay is caused by a failure to inactivate the Swe1 kinase, which blocks entry into mitosis by phosphorylating and inhibiting Cdk1 [41].
Failure of Xist RNA to coat the Xi results in failure to inactivate the chromosome (Wutz et al. 2002).
In contrast, Mad1 phosphorylation persisted long into anaphase in cdc14-1 cells, indicating a failure to inactivate the mitotic checkpoint.
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The first part of the story deals with the actual incident, which involved the failure to completely inactivate the very first lots of inactivated poliovaccine licensed for use in the United States, and the resulting epidemic of iatrogenic paralytic polio.
Therefore, the mechanism of resistance does not appear to be failure of GSI to inactivate NOTCH signaling.
In contrast, Chancey and Westley concluded that the cysteine was not required for catalysis by the yeast enzyme based on the failure of iodoacetate to inactivate the enzyme.
The failure of G407R currents to inactivate prevented the measurement of steady-state inactivation parameters.
The unusual expression of genes like SERPINB5 and S100A2 in differentiated preinvasive cells (and subsequently in tumours) may therefore indicate that a failure to appropriately inactivate stem cell-associated sequences, perhaps by epigenetic modification, is an important proneoplastic mechanism.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com