Six SNPs of ESR1 met the criteria (Additional file 1: Figure S1), but rs1801132 was excluded because of the failure of probe design.
Also, signal can be lost due to too little probe material, failure of the probe to transfer to the slide surface, pin failure in printing, variations in the slide surface, and variations in target labeling using random primers.
By way of illustration, with Roche NimbleGen's latest high-density array of 2.1 million probes, a 2% rate translates to the failure of 42,000 probes.
ESA engineers said that the landing was softer than expected judging from the 4cm depression of Philae's legs, but there was some concern over the failure of the probe's anchors to fire into the surface, suggesting that the lander may not be fixed as tightly to the comet as ESA would have liked.
Various theories were posited for the failure of the probe to make contact after the expected landing time of 02 45 GMT on 25 December.
Likewise, the failure of the probe to report decreases in cAMP concentrations indicates that concentrations of cAMP in unstimulated macrophages are at or below the lower limit of detection by Epac-camps.
However, failure of FCGR3B#1 probe in 15% of the individuals, which seemed too high a frequency for deletion, prompted us to sequence the promoter region of FCGR3B gene in 24 individuals.
For example, the failure of a probe to hybridize well to the CB4856-derived sequences might lead to false negatives.
The reason for failure of one probe (against 25S rRNA, position 1512) is unclear, although it was shorter than most of the others (see red 'x' in Figure 2B), thus it may be prudent to design probes of >22 nt length.
This in no way excuses Hergé's failure to probe critically the spirit of the time, but Tintin was, in spite of that, a kindly man, who defended the weak against the powerful.Frédéric Renard BrusselsSIR – Tintin as we know him was born during the war.
