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We show that Elg1-RLC fails to unload PCNA prior to Okazaki fragment ligation by Cdc9, and that Okazaki fragment ligation by the exogenous DNA ligase ChVLig promotes PCNA unloading by Elg1-RLC.
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Failing to unload Yasiel Puig on another team before Monday's non-waiver trade deadline, the Dodgers traveled to Colorado without their temperamental outfielder.
While PCNA failed to unload in the Cdc9-degraded cells carrying empty vector, this accumulation of PCNA on chromatin was substantially reduced when ChVLig is overexpressed.
They say the requirement fails to account adequately for loading and unloading time.
Yellow school buses will be retrofitted with a camera that snaps photos of drivers who fail to stop for a bus unloading or loading students.
Furthermore, the few studies on assembly of the human pol holoenzyme failed to account for the clamp unloading activity of the clamp loader, replication factor C (RFC).
Some media owners strip their acquisitions for the cash (Sam Zell); some fail to turn them around (Sidney Harman); some unload them when times get harder (the Bancrofts, the Grahams).
Two van loads of notes were quickly unloaded, yet even this failed to calm the panic stricken depositors.
I purchased canvas sacks to carry my groceries in (alas, I often failed to recall their existence till I was unloading full, plastic bags into my trunk).
In a week in which beleaguered Fisker Automotive failed to make a $10-million 10-millionnt, owners are loaning to unload their cars.
But when Rivera's two-seam fastball failed to run in as much as he wanted, Cabrera unloaded on it.
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CEO of Professional Science Editing for Scientists @ prosciediting.com