GST-Arg-SH3SH2 with a binding-defective point mutation in the SH3 domain (W145K) fails to bind N-WASp, while a binding-defective mutation in the SH2 domain (R198K) does not affect the affinity of GST-Arg-SH3SH2 for N-WASp.
The D119A-β3 D119A-β3 containtegrinint mutation in the containsd RGD integrin binding motif and therefore fails to bind RGD-containing ligands, such as fibronectin, vitronectin, and osteopointn [ 49, 50].
However, when tethered to an adjacent C2 domain, C2A is able to bind SNAREs [ 48], whereas tethering C2B to C2A, even a non-functional version which fails to bind lipid or calcium, enables C2B binding and penetration of membranes.
(B) PGRN fails to bind to both TNFR1 (red line) and TNFR2 (blue line) on COOHV chip.
(D) FadRk113 fails to bind to promoter regions of fabA and fabB, two UFA biosynthetic genes whereas FadRWT does.
Results of ITC experiments reveal that S100A13 lacking the C-terminal segment (residues 88 98) fails to bind FGF-1.
Spinach has been reengineered by extending its 5′- and 3′-ends to create Spinach.ST, which is predicted to fold into an inactive conformation that fails to bind DHFBI.
This chimera copurifies with FAD and displays demethylase activity, but fails to bind the partner protein corepressor of the RE1-silencing transcription factor (CoREST).
A structural feature of Vav1/2/3 is the presence of an atypical C1 domain, which possesses close structural homology to the typical C1 domains of protein kinase C but which fails to bind the second messenger diacylglycerol or the potent analogs, the phorbol esters.
There could be several reasons why AN2R fails to bind Cullin.
In contrast, CTP fails to bind to the CoaE-DCoA complex.
