Although experimental evidence indicates that ELN+ ST can produce autoantibodies [ 12], a series of large translational studies failed to demonstrate a link between synovial ELN and systemic or local RA-specific antibodies [ 5, 13, 14].
The synergistic action of two feedback loops is thought to increase robustness, although attempts to reconstitute the GEFL alone (without the post-translational feedback loop) failed to exhibit circadian rhythms.
Several attempts to express CDH in the bacterial expression system Escherichia coli failed because of the different post-translational modifications of the two domains.
Our own attempts to demonstrate the presence of the protein and its location by translational fusion with GFP [35] failed.
Notably, most previous RTPCR-based analyses have failed to take into account the possible translational control when assigning biological or clinical relevance to ERβ isoform expression.
A substantial number of therapeutic drugs for Alzheimer's disease (AD) have failed in late-stage trials, highlighting the translational disconnect with pathology-based animal models.
Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested.
In the last few decades, most translational efforts in stroke research have failed.
"The response was quite amazing, considering this was a challenging population that had pretty much failed every treatment out there," says Parekh, director of translational research in myeloma at Mount Sinai.
