As in MEFs, the MUS81 loss-of-interaction mutants failed to interact with SLX4 in co-immunoprecipitation experiments (Fig. 2C).
In yeast, the LGMD2H mutant TRIM32 D487N failed to interact with dysbindin, whereas a robust interaction between TRIM32 P130S and dysbindin was found (Fig. 1B).
Only a construct lacking the homeo-box region failed to interact with GST-PTB1, demonstrating that the interaction with PTB1 domain of FE65 takes place within the homeo-box region (Fig. 1C).
The observed interactions are specific, as H2A nucleosomes failed to interact with these two factors.
Importantly, other members of the sirtuin family failed to interact with MeCP2e1, underscoring the specificity of the SIRT1-MeCP2 interaction (Fig. S2B ).
Consistently, UXT-2M failed to interact with either NICD or RBP-Jκ, indicating the functional relevance of the interaction between UXT and NICD.
Several Dcx constructs that mimic human DCX mutant alleles failed to interact with neurabin II.
Surface plasmon resonance based studies suggested that while pep14 exhibited affinity to human and rabbit IgM, pep14 failed to interact with mouse IgM.
Yeast two-hybrid assays using serial dilutions of transformed yeast cells revealed that TTG1S215E failed to interact with TT2, while TTG1 and TTG1S215A interacted with TT2 to the same extent (Fig. 6a).
hENT1 also failed to interact with the deoxynucleoside deoxyguanosine, thymidine, deoxyuridine, or deoxycytidine (Fig. S3B).
Grb2, Gads, and PLC-gamma1 associated with LAT preferentially via different sets of tyrosine residues; however, they failed to interact with LAT mutants containing only one tyrosine.
