As in MEFs, the MUS81 loss-of-interaction mutants failed to interact with SLX4 in co-immunoprecipitation experiments (Fig. 2C).
In yeast, the LGMD2H mutant TRIM32 D487N failed to interact with dysbindin, whereas a robust interaction between TRIM32 P130S and dysbindin was found (Fig. 1B).
The observed interactions are specific, as H2A nucleosomes failed to interact with these two factors.
Importantly, other members of the sirtuin family failed to interact with MeCP2e1, underscoring the specificity of the SIRT1-MeCP2 interaction (Fig. S2B ).
Consistently, UXT-2M failed to interact with either NICD or RBP-Jκ, indicating the functional relevance of the interaction between UXT and NICD.
hENT1 also failed to interact with the deoxynucleoside deoxyguanosine, thymidine, deoxyuridine, or deoxycytidine (Fig. S3B).
Several Dcx constructs that mimic human DCX mutant alleles failed to interact with neurabin II.
Surface plasmon resonance based studies suggested that while pep14 exhibited affinity to human and rabbit IgM, pep14 failed to interact with mouse IgM.
On the other hand, 35S-Met labeled Ey with deletions of both the PD and HD failed to interact with any of the GST Ey deletion constructs (Fig. 4B, ΔPD, ΔHD*).
One of the glaucoma-associated mutants, H486R, failed to interact with CYLD (Figure 2A).
Moreover, a NOD2 construct encompassing a region immediately succeeding the CARD1 (residues 117 267) failed to interact with NLRP1.
