We found that curcumin failed to increase the promoter activity of pmrD and pmrHM in phoP- (Figure 6A) bacteria.
GATA3/R299Q overexpression failed to increase the transcription activity of the GATA responsive element.
However, IGF-1 failed to increase the level of Brk phosphorylation or activate Brk kinase activity in T47D breast cancer cells [ 25]; IGF-1 effects may have gone undetected due to high basal Brk activity in these cells (Fig. 1C).
Intriguingly, mutants of BRCA1, including BRCA1-M1775R, BRCA1-Y1853x, BRCA1 683aax, BRCand(1–683aa), and BRCA1 (1301–1863aa), not only failed to induce the basal activity of the p3TP-Lux reporter, but also failed to increase the TGF-β1-mediated activation of the reporter (Figure 1C).
As expected, TRAIL but not TNF- α failed to increase the NF- κB reporter activity after the introduction of the PKC δ D394A construct.
CR fails to increase the lifespan of SIRT1 knock-out mice, and these mice do not increase their physical activity, a phenotype typically associated with calorically-restricted mice [ 25, 26].
H2O2 treatment failed to increase ENaC activity in the presence of Fulvene-5.
10.7554/eLife.06120.018 Figure 7. Famutantstants found in AI patients fail to enhance Fam20C activity, and WT Fam20A is able to increase the activities of Fam20C mutants.
As observed previously, expression of wild-type HPV16 E7 reproducibly results in increased activity from the p73 promoter, but mutants E7Δ21-35 and E7 Gly 24, which do not associate with pRb, failed to increase activity from the p73 promoter.
