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In addition, qRT-PCR failed to detect the expression of hsa-miR-122 and hsa-miR-502-3p hsa-miR-502-3p hsa-miR-502-3p hsa-miR-502-3p
For instance, the array failed to detect the expression of miR164a,b and miR396, whose expression in inflorescence was confirmed by both ASRP database and RT-PCR.
Furthermore, even though we failed to detect the expression of the six transcripts on the arrays, we recovered the expression of the two transcripts by RT-PCR.
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While our experimental evaluation of computationally predicted tissue-specific isoforms revealed a high success rate in confirming the expression of these isoforms in the respective tissue, the strategy frequently failed to detect the expected restricted expression pattern.
Protein levels of hepatic AMPKα1 or α2 were failed to detect, whereas the expression of AMPKα2 or α1 was not changed, similar with the previous report20.
We further used RT-PCR to detect the expression of TNF-h (Fig. 3B).
Allele-specific real-time PCR was employed to detect the expression of H3mm753.
Quantitative RT-PCR analysis also failed to detect any expression of the endogenous OCT4 and NANOG, two essential markers for human pluripotent stem cells (Fig. 2C).
Other recent RNA profiling experiments also failed to detect differential expression of R genes [ 90, 91].
In contrast, we failed to detect any expression of TNAP in resting chondrocytes or after stimulation with TGF-β1.
While we detected increased protein expression of BMP-7 and BMPR-II in TDSCs (CI) compared to TDSCs (HT), we failed to detect increased expression of their corresponding mRNA.
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