Sentence examples for failed to detect endogenous from inspiring English sources

Parallel experiments performed on the serum of healthy donors failed to detect endogenous morphine (n = 3; data not shown).

In western blots, the anti-DSas-6 antibodies failed to detect endogenous DSas-6, but specifically recognized the GFP-DSas-6 fusion protein.

We could not directly assess Cyclin D1 phosphorylation in Tg Dyrk1a embryos because pT286 antibodies failed to detect endogenous phosphorylated Cyclin D1.

While the antibodies recognized recombinant protein and bZIP63 in the ox extracts well, they failed to detect endogenous levels of bZIP63, possibly due to the low abundance of the protein (not shown).

In contrast, our own studies have failed to detect interaction between endogenous Dnmt3L and Ezh2 by co-immunoprecipitation using an anti-Dnmt3L antibody.

Quantitative RT-PCR analysis also failed to detect any expression of the endogenous OCT4 and NANOG, two essential markers for human pluripotent stem cells (Fig. 2C).

We attempted to compare the degree of LCN7 protein overexpression to basal LCN7 expression using commercially available LCN7 antibodies, however these antibody preparations demonstrated significant non-specific binding and failed to detect overexpressed protein much less endogenous LCN7 (data not shown).

However, despite the activation of miR-16-2 probservedbserved in the luciferase assay, we failed to detect any appreciable transcriptional induction of the endogenous miR-16-2 gene.

This localization may result from protein overexpression because we failed to detect similar localization in yeast strains with their endogenous BUD3 gene tagged by GFP (data not shown).

Polyclonal Sigma Prestige antibody failed to detect TKTL1 in all cell lines tested, indicating a lack of endogenous TKTL1 protein.

However, this is unlikely because we failed to detect the depressed perception of dyspnea which is also inhibited by endogenous opioids [ 38].