Indeed, we found that, while expression of full-length IWR1 from a plasmid rescues RNAPII and III localization in iwr1 Δ, expression of partial or complete deletions of the NLS sequence failed to improve localization of Rpb1–, Rpo31–,or Rpc37 GFP fusion.
In contrast, similar SMN constructs aa1-1944 and aa190-294), were shown to have a pan-cellular localization in COS-1 cells, however, both constructs failed to localize to nuclear bodies [100].
The fluorescent complexes containing either mutant protein, however, failed to demonstrate localization at the cell leading edge or serum-stimulated membrane translocation.
While re‐expression of wtPRG‐1 in PRG‐1‐deficient neurons was reported to rescue the frequency of miniature excitatory postsynaptic currents (mEPSC) to wild‐type levels in hippocampal CA1 pyramidal cells (Trimbuch et al, 2009), PRG‐1R346T, albeit present at the proper dendritic localization, failed to achieve this functional rescue.
CpoB also failed to localize following inhibition of PBP3 with aztreonam, indicating that its localization also requires ongoing septal PG synthesis.
If MCAK localization depends on its interaction with TIP150, the phospho-mimicking mutant that failed to localize to the plus end could disrupt the MCAK TIP150 interaction.
However, although this domain contains two putative nuclear localization signals, mutation of these motifs failed to prevent nuclear localization [ 14].
Although in silico protein localization prediction failed to identify AtCuAO2 as a putative peroxisomal protein, the localization pattern observed using in vivo targeting analysis of CuAO2-YFP fusion protein strongly suggests that it is also a peroxisomal protein.
This localization may result from protein overexpression because we failed to detect similar localization in yeast strains with their endogenous BUD3 gene tagged by GFP (data not shown).
