It failed in sequences where there was insufficient contrast between the ocean and the target and thus fails to pick up specific motion of the target, when the target moves too slowly and is thus considered part of the background and when there is a lot of glint in the scene.
In other words, 7 were validated by only sequencing but failed in SNaPshot, and 13 by only SNaPshot but failed in sequencing.
Sequencing results confirmed 91 PCR products to be polymorphic, and seven (P008, P010, P025, P050, P055, P058, and P062) of the remaining PCR products that failed in sequencing appeared polymorphic based on the existence of multiple DNA bands on agarose gels.
The PCR products that were failed in direct sequencing were cloned into pGEM-T Easy Vector (TaKaRa) and at least three independent clones were sequenced.
Protocol-required system-related event notifications were end of service, elective replacement indication (ERI), atrial impedance <250 or >1500 Ω, ventricular impedance <250 or >1500 Ω, daily shock impedance <30 or >100 Ω), and ineffective ventricular maximum energy (30 J) shock (notified if first shock failed in a given episode sequence).
Re-sequencing failed in 25 individuals (overall sequencing success rate of 98.5%) so that ultimately we had HVSI data from 835 case-father pairs.
The numerical analysis did not consider the debilitating effects of the anchor hole on the steel plate and therefore predicts that beam B-5-2 fains in the sequence of yielding of reinforcing bar, yielding of steel plate, and then crushing of concrete.
Traditional strategies like genetic screening, microarray and bioinformatics prediction have miserably failed in species where genome sequence is not available such as stevia.
In conclusion, our tracker performs well in all the 12 sequences while other 9 state-of-the-art trackers fail in some sequences.
Capillary sequencing failed in ∼20% variants.
