Suggestions(5)
Exact(7)
In summary, loss of Rho function or failed expression of the pre-TCR complex in Rag2−/− null mice block T cell development at a common stage.
In several cases (ELF1, Fli1, Ldb1, BC031407, Nfkb1 and RelA-p65) truncating the proteins to minimise the molecular weight and the numbers of low complexity regions and contiguous hydrophobic amino acids made the difference between failed expression and good soluble protein expression.
In this respect, microarray analysis of Rag2−/− pre-T cells revealed decreased expression of TCR-β subunits as a major defect (Supplementary Fig. 4) and failed expression of the TCR-β subunit is indeed responsible for the DN3 developmental block in Rag2−/− mice.
The loss of DUSP expression is thus almost certainly a secondary consequence of failed expression of Egr molecules.
The reasons for failed expression included insoluble production as inclusion bodies or toxicity resulting in death of the expression cultures (Additional file 1: Table S1 S3).
Rag2−/− mice also block thymus development at the DN3 stage (Figs. 1A and B) due to failed expression of the pre-TCR complex.
Similar(53)
For those cases on which SISH failed, the expression was based on IHC.
Four samples failed gene expression profiling probably due to low yield or low quality (see Additional file 1).
In contrast, a decrease in SMAD3 expression failed to alter SPARC expression.
The regulation of leptin was mainly Hif-2α-dependent, because the knock-down of Hif-1α expression failed to affect leptin expression.
It is known that the changes of local appearance (e.g., eyes, nose, mouth) are usually related to facial expression variations; meanwhile, the global features that are related to the whole facial image may fail on expression analysis.
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Justyna Jupowicz-Kozak
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