Exact(26)
The flanking regions of the remaining three failed assays did not contain predicted exon-intron boundaries or secondary SNPs.
In total, 2,605 of the 3,498 "failed" assays corresponded to SNPs and 893 to indels, whereas 1,162 of the 3,181 monomorphic loci corresponded to SNPs and 2,019 corresponded to indels.
When these failed assays were excluded from the comparison between HRM curves and sequencing, sensitivity increased to 92%, with an accuracy of 80%.
Initially, these data were considered failed assays.
After removing failed assays, 34.398 duplo genotyping assays remained.
Any failed assays were excluded from the analysis.
Similar(34)
A set of 28 SNPs failed assay design, leaving us with 341 SNPs for genotyping in our study (Table S1).
Some 1,143 of the 3,769 SNPs that failed assay conversion in manufacturing were tagged by at least one passing SNP already on the array (CEU r2> = 0.8).
Of the six candidate SNPs from the Oregon-SUDS GWAS, one failed assay design, and 1 was out of Hardy Weinberg equilibrium (HWE) (P<0.001) in both whites and blacks from the ARIC/CHS cohort.
The third failed assay was cr_lrc282.
This failed assay was not repeatable since the RNA sample in question was spent.
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