Sentence examples for failed alignment from inspiring English sources

After removing sequences that failed alignment and splice site validation criteria (see Methods), 62,275 ESTs that align to bulk (non-rDNA) MAC chromosomes remained.

As previously reported this situation can result in a failed alignment as a target read r looks too dissimilar to any known target genomic sequence [ 13].

Overall, 59'316 sequences were validated on one or the other genome (arising from 48'217 different loci), 10'208 were found in the genome but failed alignment validation due to the PASA options used (see above), and 844 sequences did not align to the genome at all.

Lowering the identity threshold below which alignment is refused by PyNAST to 0.0001 reduced the number of failed alignments.

If the sequence failed to alignment, two more nucleotides at 3' end were deleted.

Automated alignments have failed to align the region N-terminal to the equivalent of Ser806 critical for ATP binding.

The vast majority of ESTs derived from genome regions not represented in our assembly would be expected to fail both alignment criteria of greater than 90% identity over greater than 95% total sequence length, but none of our EST reads did.

Reads that failed in the alignment procedure were extracted from alignment files using SAMtools (version 0.1.19); and assembled de novo in Trinity (version 2.06) [ 49] with default parameters.

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In addition, 816 segments with sequence in both genomes failed to align with LASTZ and also failed to produce consistent alignments with different Smith Waterman alignment implementations.

We also examined the ESTs that failed our strict alignment criteria.