Once those factors were added in, the report said, the carbon dioxide emitted by trains was far higher than expected.
The methodology was fully applied on the territory of Moscow, the analysis becomes more detailed on each stage of the process and new factors were added.
To explore the effects of environmental factors on species-species association networks, environmental factors were added to these networks (Supplemental Figs S3 and S4, in which red lines signify positive correlations and black lines signify negative correlations).
The following factors were added to DKSFM: human recombinant EGF (10 ng/mL; Peprotech, Rocky Hill, NJ, USA) and cholera toxin (CT) (100 μg/mL; Funakoshi Co., Tokyo, Japan) as previously reported.29 Differentiated cells were examined by immunostaining.
Next, the enabling factors were added, and then the need factors were added to assess any potential mediating effects.
As an estimation, the resulting factors were added to the LCIA method.
When the enabling factors were added to the model (Model 2), there were some mediating effects among the predisposing factors.
When all factors were added to the culture medium, the cells exhibited features that closely resembled human adult hepatocytes (Chivu et al., 2009).
No exogenous growth factors were added.
Candidate factors were added 2 hours after plating in all experiments.
No exogenous cytokines or growth factors were added to aid cell differentiation.
