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An analogous experiment, in which S. oralis isolate SV11 was incubated with a solution of purified human plasminogen demonstrated its direct interaction with the bacterial surface indicating that the binding does not depend on other factors from human plasma (Fig. 1).
To determine the effect of SNARE cleavage by retargeted BoNT/B, we measured the release of cytokines and other factors from human BMDMs.
Protein array was used to evaluate levels of angiogenic and growth factors from human islet-BM co-culture and islet-only culture media (once a month from day 1 to 210).
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Human KLF4 was first identified using a DNA probe containing the zinc finger region of human erythroid Krüppel-like factor from human umbilical vein endothelial cell cDNA library [ 15].
In the human colon carcinoma cell line, paracrine stimulation by a soluble factor from human colon connective tissue was involved in inducing the expression of the MUC1 mucin in vitro (Irimura et al, 1990).
Siman et al. (2004) reported that tau BDP (breakdown product) can be detected in neuronal culture media following neurodegenerative challenge and in CSF (colony-stimulating factor) from human TBI patients.
Independently, researchers at the Snow Brand Milk Products Co. (Sapporo, Hokkaido, Japan) reported their discovery of an identical molecule [ 9] using the standard approach to test the Rodan Martin hypothesis of purifying a factor from human embryonic fibroblasts that inhibited osteoclastogenesis.
The migration activity of the outgrown cells was analyzed in response to distinct growth factors (IGF-1 and PDGF-BB) as well as the cocktail of factors released from human cartilage after in vitro traumatization.
Soluble factors released from human prostate carcinoma cells significantly increased viability of naïve bone marrow monocytes, as well as osteoclastogenesis from precursors primed with receptor activator of nuclear factor κ-B ligand (RANKL).
To answer this question, we examined the concentrations and effects of angiogenic factors released from human HCC cell line, HuH7, in the presence and of absence of IFN and 5-FU and their combination.
No murine MyoD transcripts were detected indicating that the conversion is probably driven by myogenic transcription factors from neighboring human nuclei within the same cytoplasm.
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