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Cells to be tested are placed in the upper chamber, and the migration-promoting factor to be tested for chemotaxis is placed in the lower chamber.
For each of the 10 pairs (A-J), the two competing isolates had (nearly) identical AFLP profiles and identical virulence factor profiles, except for the virulence factor to be tested (marked in bold).
Given that four of the SNPs composing the CRC-predisposing combinations are related to inflammation, the most relevant "environmental" factor to be tested here would have actually been NSAIDs treatment.
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The optimal design, given the number of factors to be tested and the constraint of available ship time (100 days) and resources needed to process samples, was for 12 control and 12 impact plots each measuring ∼2.8 km × 1.2 km.
This limited the number of factors to be tested and the precision of our results.
Then fresh medium was added along with the growth factors to be tested for the indicated time periods.
Then fresh medium was added along with the growth factors to be tested and methyl-[H]-thymidine (0.2 μCi/mL, 25 Ci/mmol).
The area under receiver operating characteristics curve (AUC) is calculated to evaluate the performance of the proposed algorithms under different settings of the 3 key factors to be tested.
For transient transfection, a luciferase reporter was co-transfected into HEK293 cells with expression plasmids for β-gal and factors to be tested using FuGENE HD reagent (Roche, Basel, Switzerland).
Because species and populations within each species complex cannot be regarded as independent data points, we also performed a comparative analysis using Generalized Estimating Equations (GEE), which allows multiple factors to be tested simultaneously while controlling for phylogenetic effects [ 109].
If the factor is to be tested for chemokinesis, it is added at the same time to both upper and lower chambers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com