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For determination of the cytotoxicity of CAMP factor, cells (1×105/well) were incubated in a 96-well plate with recombinant CAMP factor or GFP in a 1% FBS-medium for 18 hr.
To eliminate the influence of growth factor, cells were also cultured in ordinary plates, which retained adherent growth and were named as Conditioned T24 cells.
Without either factor, cells destined to become pluripotent in the blastocyst never acquire the ability to generate embryonic lineages (Mitsui et al, 2003; Nichols et al, 1998; Silva et al, 2009).
In Drosophila, the amount of survival factor cells compete for is often not limiting, but cell selection still occurs because cells can compare their fitness directly thanks to fitness indicator proteins.
The final calculation for cell count in the stock solution was carried out using the following formula: cells/ml = (dilution factor) (cells counted) (calibration constant)/number of squares containing cells counted [ 31].
For synchronisation with α factor, cells were grown at 25°C to an OD: 0.2 0.4 and cell synchrony was monitored my bright field microscopy over 2 3 h to achieve at least 90% arrested cells.
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A value of P < 0.001 was considered significant (effect factor: cell type specification).
Overall, 39 combinations of these three factors (cells) described 93% of the New Zealand landscape.
Tissue engineering requires growth factors, cells and a scaffold to permit effective tissue regeneration.
Bioprinting enables the deposition of various biologics including growth factors, cells, genes, neo-tissues and extra-cellular matrix-like hydrogels.
After removing the α-factor, cells were immediately plated on MAB6 plates and irradiated with 15 J/m of ∼254 nm UV, using a TL-2000 UV Translinker.
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