Post-translational modifications to histone proteins, including acetylation, methylation, phosphorylation, sumoylation and ubiquitina-tion, regulate transcription factor access to gene-encoding regions of DNA and facilitate gene transcript elongation [ 7].
In qm mutant egg-chambers, the ring canals are clustered together centrally, and Dynein heavy chain (Dhc), which accumulates at MT minus-ends, is enriched adjacent to the ring canal aggregates, supporting the idea that ring canals can nucleate nurse cell MTs to facilitate the transcript transport from nurse cells to the oocyte (Clark et al. 2007; Mische et al. 2007).
Compensation of the ion regulatory apparatus might additionally take place on the post-transcriptional level or is facilitated by transcripts not included on this microarray.
Additional applications facilitating SNP and transcript discovery, alternative statistical methods for quantifying gene expression, etc. are also pursued at pilot scale to inform future array designs.
Such studies further indicated that a single RNA sequence element, the GLS, mediates both transport steps by facilitating association of gurken transcripts with a cytoplasmic dynein motor complex.
Our collection shows enhanced enrichment for full-length transcripts thus facilitating downstream functional analysis of newly discovered genes.
This reduced the complexity of the transcript population, facilitated the identification of differential transcript processing, allowed clustering of similar expression profiles and simplified comparisons with expression data generated using alternate technology platforms.
It was the first mobile RNA identified and CU-rich sequences in its transcript facilitate binding to CmRBP50, a PTB protein of pumpkin.
In addition, the high resolution of RNA-Seq can facilitate discoveries of transcript dysregulation that have been missed by previous technologies.
The mapped read counts for each gene were normalized for RNA length and for the total read number in the lane according to reads per kilobase of exon model per million mapped reads (RPKM), which facilitates comparison of transcript levels between samples [ 45].
