The plasmid pGIL001 was constructed to facilitate replacement of the KanMX4 cassette with the URA3 gene.
Our observations suggest that somatic cells destined to proliferate extensively in tissue homeostasis and, upon subsequent tissue expansion, undertake this role by adopting selective segregation of their original DNA in order to facilitate replacement or expansion of committed tissue cells that are devoid of genomic defects due to DNA replication.
The application of a variable-speed drive can enable the use of locally-manufactured pumps which are not necessarily designed specifically for photovoltaic applications, thus facilitating their replacement and maintenance.
Facilitating the replacement of A opposite 8-oxoG with a C, MYH produces the preferred substrate for the 8-oxoG directed DNA glycosylase OGG1.
TALENs can also be used to generate heritable knock-in of large DNA fragments by homologous recombination with double-stranded donor DNA (Zu et al., 2013), facilitating gene replacement of knocked-out genes with fluorescent reporters or the creation of oncogenic point mutations within targeted genes.
Enamelysin (matrix metalloproteinase [MMP]) 13 degrades amelogenin, 17 eliminates enamel matrix proteins, 18 facilitates replacement of the organic matrix with minerals, 19 and processes amelogenin during the secretory stage, 20 although bone resorption in tooth eruption is seen associated with the expression of osteoprotegerin.
The earliest forms of NC were developed in the 1950s when the movements of the axes of machine tools were assigned numerical values to facilitate the replacement of handwheels and dials by control logic.
But while the mistreatment of Dust Devil was disheartening, it was on the set of the ill-fated Island of Dr Moreau remake that Stanley would really come a cropper, finding himself at loggerheads with star Val Kilmer who promptly facilitated his replacement by ageing hack John Frankenheimer.
"The financial stability plan outlined last week ensures that there is capital available where it is needed, and that it is provided in a way that will facilitate the replacement of public support with private capital," said a spokeswoman for Mr. Geithner, Stephanie Cutter.
It would also deter CEOs from leaving unpleasant surprises for their successors and give them more incentive to orchestrate or facilitate their replacement by strong ones.
